Output format

Mutation candidates are sorted by Read frequency and Map quality.

Chr	Position	Reference allele	Mutation allele	MapQ	Reads	Read frequency	Mutation type	*Note1	*Note2
chr01	1520327	G	A	222	35	100	NON_SYNONYMOUS_CODING\|MISSENSE\|Ctt/Ttt\|L322F\|mip1\|SPAC57A7.11.1
chr01	682991	CTT	CT	214	22	100	FRAME_SHIFT\|\|-/-\|-40\|snu23\|SPAC22F3.11c.1	chr01:682993:TCCCC:TCCC:2

Mapping ratio
Coverage depth

column 1:Chromosome
column 2:Position
column 3:Reference allele
column 4:Mutation allele
column 5:Map quality
column 6:Number of Reads mapped in this position by mapping.
column 7:Read frequency: Ratio of reads with mutation allele among all mapped reads in this position.
column 8:Mutation type that annotated by SnpEff (http://snpeff.sourceforge.net/).
column 9:Note1. Information about a neighboring variant in the same gene in the parental strain (DuM).
column 10:Note2. *Mark means that existence of a neighboring variant in the same gene in the mutants.

Column 9 "Note1" and column 10 "Note2" is available when you set a pair of mutant and parental data.